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How an Overnight COVID-19 Test Took 35 Years to Invent

By Shlomo Maital

Dr. Fang  Zhang

   As Darwin observed, when he was praised for his breakthrough: Scientists stand on the shoulders of giants. Now, a new genetic test for COVID-19 may be as quick, simple and cheap as a self-administered pregnancy test, with two lines on a slip of paper.

     Here is the story. Let’s begin by noting that the hero is ethnic Chinese, Fang Zhang, a researcher at MIT’s Broad Institute. With massive anti-Asian and anti-Semitic hatred filling the Internet, it is fitting the hero should be named Zheng.

     Chapter One. Some 35 years ago, a biologist named Kary Mullis invented PCR. Polymerase chain reaction (PCR) is a method used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample. This allows scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. For example, suppose you are looking for the presence of the genetic material of a virus. You take a swab, use PCR, make millions of copies of the stuff, and then it becomes easy to detect it in a test tube.   Mullis won the Nobel Prize for this discovery, in 1993.

     Chapter Two. CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats. (A palindrome is a word or phrase that reads the same forwards and backwards, e.g. a man a plan acanal panama. ) The discovery of clustered DNA repeats occurred independently in three parts of the world. The first description of what would later be called CRISPR is from Osaka University researcher Yoshizumi Ishino and his colleagues in 1987.

     Say you want to modify a specific gene – snip it out, replace it, test it, etc. First, you ‘tag’ it with a molecule (like putting a big road sign, “HERE I AM!”, on it). Second, you attach an enzyme to the tag. The enzyme cuts the CAN right at that spot!   You can then replace the faulty or offending gene with a different improved one.

     Chapter Three. MIT. Dr. Zhang and other researchers have retooled CRISP-R. They use it not to snip out a gene, but to give a signal that the enzyme has reached its target – a piece of genetic material that is unique to COVID-19. When this happens, a screaming signal is emitted, say, figuratively, a bright Day-Glo sign saying, Yikes, it’s the novel coronavirus!  

       Chapter Four. Translate the complex lab procedure to a simple cheap test. Take samples from a person’s throat and nose. Put it into a test tube with chemicals that tear open viruses.   Use an eye dropper to put some of the liquid into a second test tube containing CRISPR. Put the test tube in hot water, at 140 degrees F. (80 C.). Stick a piece of paper into the tube. If two lines appear: COVID-19 is present.

     The test worked on 12 patients, and can be simplified greatly and produced at $6 per test. This may enable massive population-wide testing, that can help open societies and economies without a massive second-wave of plague.

       Classic scientific breakthrough: 35 years, and an overnight breakthrough!




CRISPR Will Change Our Lives

By Shlomo Maital

   Some time ago, I wrote about a technology known as Blockchain that will undoubtedly change our lives – and has already. Blockchain is simply a way to record transactions, that is secure, unhackable and ‘disintermediated’ (no need for banks or other financial middlemen). It is now widely used to create digital money.

   Now comes CRISPR. It stands for Clustered Regularly Interspaced Short Palindromic Repeats.     What is it? Simply, a way of editing genes – like we edit blogs or emails. Erase this group of words. Replace it with another, better one.   In the case of DNA: “Erase” (snip away) this (bad) piece of DNA, a gene that causes problems – and replace with a ‘good’ gene, that will not cause disease or problems.

   A palindrome is a word that reads the same forward and backward.   E.g. “civic”. So pieces of DNA are inserted into longer pieces, such that the inserted pieces read the same forward or backward, so it doesn’t matter which way they are inserted. The method originated with studying how viruses ‘snip’ DNA – and using viruses to do the same in constructive way.

     We now know the genetic causes of many diseases. But until now we have not had the ability to repair bad genes. Now CRISPR makes it possible. This will create an entirely new branch of medicine, immunotherapy, in which gene therapy is used to both treat illness, when identified, and mainly, to prevent it — an individual can now have his or her genome analyzed, and potential ‘bad’ genes identified. No point in doing this, so far, because there was no real way to ‘edit’ bad genes. Now, with CRISPR, there is.

     I would like to mention one of the scientists responsible for CRISPR, the young MIT scientist Feng Zhang. He was born in China and is only 36; he does research at the famous Broad (pronounced Brode) institute in Cambridge, MA. Increasingly bright foreign students are encountering US visa problems and are going elsewhere, e.g. Canada. It is America’s loss.

Blog entries written by Prof. Shlomo Maital

Shlomo Maital